| 1. | Breeding of high - yield l - isoleucine producing strain by protoplast fusion
异亮氨酸高产菌的原生质体融合育种 |
| 2. | Mesophyll protoplast fusion of solanum tuberosum and solanum chacoense and their somatic hybrid analysis
马铃薯栽培种与野生种叶肉细胞融合及体细胞杂种鉴定 |
| 3. | For this it is inconvenient or disadvantageous to exploitation and applications of bifidobacterium . utilizing the technology of protoplast fusion , facultative lactobacillus ' s aerotolerant gene was integrated
本实验以兼性厌氧乳杆菌作为遗传物质的供体，应用原生质体融合技术来改良双歧杆菌菌种厌氧特性。 |
| 4. | Somatic hybrid cells were obtained between ethionine resistant cell line of astragalus adsurgens pall , and agrobacterium / " / z / zogerae . s ' - transformed cell line of medicago saliva l . using protoplast fusion by peg method
通过peg诱导原生质体融合和杂种细胞的筛选培养，首次得到沙打旺( + )紫花苜蓿的属间体细胞杂种。 |
| 5. | Interspecific protoplast fusion between aspergillus terreus t - 730 , an itaconic acid producer , and aspergillus niger ni - 5k , a glucoamylase producer , was done to breed new mold producing itaconic acid from starch . the ni - 5 strain was induced with antibiotic - a , which became a drugresistant strain ni - 5k
选择衣康酸高产菌株牺土曲霉aspergillusterreust - 730和柠檬酸高产菌株黑曲霉aspergillusnigerni - 5 ，以抗菌素a对黑曲霉ni - 5进行诱导培育，形成遗传稳定的抗药性菌株ni - 5k 。 |
| 6. | High active phytase producing fungs - aspergillus niger were selected by mutiple uv mutation , the definitions of phytase activity were analysised and the measure wavelength of the enzyme was modified , the factors that influence the preparation of protoplast were investigated . base this , use protoplast - uv mutation and protoplast fusion to filter phytase produce asp . niger
本文以多轮紫外诱变为主线技术筛选植酸酶的黑曲霉高产菌，分析比较植酸酶酶活定义和植酸酶酶活测定方法并修正其测量波长，考查黑曲霉原生质体制备的影响因素，并在此基础上，用原生质体紫外复合诱变和原生质体融合技术筛选植酸酶的黑曲霉产生菌。 |
| 7. | Irradiation or heat treatment protoplasts fusion were developed . equal protoplast suspensions ( containing about 108 / ml protoplasts ) of each strain were mixed and washed by centrifugation , then the pellets were resuspended in peg4000 solution ( 35 % ) at 37 ? for 2min . after centrifugation and resuspension , protoplasts were diluted and plated on regeneration medium
其次，确定了原生质体融合的基本条件及灭活原生质体融合的条件：等量混合两亲株原生质体或用热灭活(或紫外灭活)的原生质体( 10 ~ 8个ml ) ，用35的peg4000在37处理2min后，稀释，涂布再生，可获得融合率在50左右的融合子。 |
